Western blot analysis of SP140 in extracts of various cell lines. Samples were incubated with SP140 Polyclonal antibody (Product # PA5-110417) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of SP140 in mouse spleen, mouse thymus, rat thymus. Samples were incubated in polyclonal SP140 antibody (Product # PA5-110417) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supplier Page from Thermo Fisher Scientific for SP140 Antibody